This year we are highlighting some of the work we’ve done in the past year.
The first poster provides an overview of how changes to our PacBio pipeline have increased our sequencing yields and read lengths, resulting in finished, high-quality microbial genomes, assembled using only PacBio data.
The second poster demonstrates how Next Gen sequencing can be used to investigate host and pathogen associations in cases of pulmonary non-tuberculous mycobacterial (PNTM) infections.
It has been a busy January for our PacBio RSII instrument. We are excited to report a new record yield from a single SMRT cell – 896,457,524 passed filter bases! It seems we are not far off from hitting 1 G.
Some more stats from this cell:
Mean Read Length: 8391 bp
P50 Subread Length: 6187 bp
P90 Subread Length: 12314 bp
P95 Subread Length: 14032 bp
Maximum Subread Length: 24585 bp
We have come a long way in the past year. Here is a comparison of yields and mean read lengths of our top 20 SMRT cells in January 2013, compared to our top 20 SMRT cells so far in 2014:
The increases in both SMRT cell yields and read lengths are making PacBio an attractive option for sequencing and finishing microbial genomes. We are excited to see where 2014 will take us!
The GRC, which offers services from sequencing library prep through genome assembly and downstream analysis, is generating complete bacterial genome sequences and methylation profiles using PacBio SMRT sequencing on the RS II. Several advancements in the library prep, sequencer, sequencing protocols, and data analysis software have all contributed to this.
To learn more about these breakthroughs and other emerging applications of SMRT sequencing, please read the PacBio Core Lab Profile showcasing the research performed at GRC and IGS here.
GRC and IGS offer not only cutting-edge sequencing, but a complete menu of services including assembly, annotation, and custom analyses. For more information about services offered, visit our Laboratory Services and Analysis Services pages. Please contact us if you have any questions.