We are excited to announce that our HiSeq 4000 is here and running. We are off to the races! The run time for two flow cells is down to 3.5 days, which will allow us to complete projects faster than ever. Each dual flowcell run can sequence up to 12 human genomes, 180 exomes, or 100 transcriptomes.
Our first flow cell, a PE150 run, produced 2.9 billion clusters and more than 850 Gb of data.
Here are some basic stats from our first flow cell:
| Lane | Total Reads | Total Bases | %PF | %Q20 | %Q30 |
| 1 | 727,970,622 | 109,923,563,922 | 75.41 | 94.42 | 87.53 |
| 2 | 777,363,006 | 117,381,813,906 | 80.53 | 96.27 | 91.53 |
| 3 | 754,973,540 | 114,001,004,540 | 78.21 | 96.24 | 91.49 |
| 4 | 753,317,088 | 113,750,880,288 | 78.03 | 95.86 | 90.36 |
| 5 | 749,170,142 | 113,124,691,442 | 77.61 | 96.21 | 91.07 |
| 6 | 684,821,518 | 103,408,049,218 | 70.94 | 96.04 | 90.89 |
| 7 | 665,485,116 | 100,488,252,516 | 68.94 | 95.65 | 90.25 |
| 8 | 602,875,822 | 91,034,249,122 | 62.45 | 95.17 | 89.38 |
Do you have a project that could benefit from the combination of increased output and read length the HiSeq 4000 offers? If so, please contact us. We will discuss the details of your project with you and guide you through the process of planning your sequencing experiments.