This year we are highlighting some of the work we’ve done in the past year.
The first poster provides an overview of how changes to our PacBio pipeline have increased our sequencing yields and read lengths, resulting in finished, high-quality microbial genomes, assembled using only PacBio data.
The second poster demonstrates how Next Gen sequencing can be used to investigate host and pathogen associations in cases of pulmonary non-tuberculous mycobacterial (PNTM) infections.
At AGBT a couple of weeks ago, I presented a poster with an overview of methods developed by GRC members to sequence and assemble viral genomes from clinical samples. To view the poster, follow the link below:
IGS also presented a poster about custom capture at this year’s AGBT meeting. The poster below presents data demonstrating that custom capture can be an effective way to sequence entire genomes of obligate intracellular parasites that cannot be grown independently, including such organisms isolated form field samples.
One of the posters IGS presented at AGBT 2013 involved a thorough comparison of sequencing platforms and assemblies strategies across 5 microbial species that vary in both genome size and GC content. The conclusions from this study aim to inform future large-scale microbial projects and aid in efficiency and project design.
Please click on the link below to view a PDF image of the actual poster.