GRC a PacBio Certified Service Provider; Co-sponsoring SMRTest Microbe Grant Program at ASM 2015

We are pleased to announce that the GRC is the first PacBio certified service provider on the East Coast. This recently announced program is a partnership between PacBio and select sequence providers who have completed the certification process and offer the highest quality sequencing and analysis services using the PacBio technology. We offer a full range of PacBio services, including whole genome sequencing, transcriptome sequencing via Iso-Seq, targeted amplicon sequencing, and other customized applications. Our analysis team has expertise in genome assembly and annotation, variant analysis, transcriptome analysis, and base modification detection. We look forward to continuing our strong partnership with PacBio and offering the highest quality sequencing and analysis to our customers and collaborators.

As part of this new partnership, the GRC is proud to co-sponsor the SMRTest Microbe Grant Program. One lucky winner will receive sequencing and analysis services from the GRC. To enter, submit a short grant application detailing your project and how it would benefit from the long reads and high consensus accuracy of SMRT Sequencing. The deadline for submissions is June 27, 2015.

For more information on our full range of sequencing and analysis services, visit our Laboratory Services and Analysis Services pages. Please contact us if you have any questions or would like a quote.

If you are attending ASM 2015 next week, please stop by the IGS booth (#776) to learn more about the grant program and all of our sequencing and analysis services. See you in New Orleans!

HiSeq 4000 Update

We are excited to announce that our HiSeq 4000 is here and running. We are off to the races! The run time for two flow cells is down to 3.5 days, which will allow us to complete projects faster than ever. Each dual flowcell run can sequence up to 12 human genomes, 180 exomes, or 100 transcriptomes.

Our first flow cell, a PE150 run, produced 2.9 billion clusters and more than 850 Gb of data.

Here are some basic stats from our first flow cell:

Lane Total Reads Total Bases %PF %Q20 %Q30
1 727,970,622 109,923,563,922 75.41 94.42 87.53
2 777,363,006 117,381,813,906 80.53 96.27 91.53
3 754,973,540 114,001,004,540 78.21 96.24 91.49
4 753,317,088 113,750,880,288 78.03 95.86 90.36
5 749,170,142 113,124,691,442 77.61 96.21 91.07
6 684,821,518 103,408,049,218 70.94 96.04 90.89
7 665,485,116 100,488,252,516 68.94 95.65 90.25
8 602,875,822 91,034,249,122 62.45 95.17 89.38

 

Do you have a project that could benefit from the combination of increased output and read length the HiSeq 4000 offers? If so, please contact us. We will discuss the details of your project with you and guide you through the process of planning your sequencing experiments.

Over 100 Publications Strong!

Less than eight years after beginning in an empty lab, our sequencing and analysis has contributed to more than 100 peer-reviewed publications. The first, published in Nature, was a comparison of the gut microbiome in obese and lean twins (DOI: 10.1038/nature07540). Since then, data generated by the GRC have been used for many other types of studies. Check out our list of publications.

If you have a publication resulting from data generated by the GRC that isn’t listed here, please let us know!

Thanks to all of our collaborators on these exciting projects for making the GRC a success. We look forward to the next 100 publications!