The latest paper by Tracy Hazen, “Transcriptional Variation of Diverse Enteropathogenic Escherichia coli Isolates under Virulence-Inducing Conditions”, confirms that the genomic diversity that has been recently identified among the EPEC pathovars corresponds with differences in their global transcription. This study expands upon previous work (including her 2015 paper “RNA-seq analysis of isolate- and growth phase-specific differences in the global transcriptomes of enterophatogenic Escherichia coli”), that showed that even well-studied EPEC strains exhibit isolate -, medium-, and growth phase-specific differences in their global transcription, including the expression of key virulence factors. The current study, by using EPEC isolates from more recent outbreaks and including those from three E. coli phylogroups, explores the transcriptional and biological diversity of a modern, disease-causing pathogen. Nine EPEC isolates from eight phylogenomic lineages and three phylogroups were selected for unbiased global RNA-seq following growth under a single set of virulence gene-inducing conditions. The results showed both lineage- and isolate-specific transcriptional profiles. This previously unexplored diversity within a single E. coli pathotype may contribute to isolate-specific variability in EPEC clinical presentation